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KMID : 0374919950160030513
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Inje Medical Journal
1995 Volume.16 No. 3 p.513 ~ p.518
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Properties of the Purified Hemolysin from a Psychrophile Isolated from Sea Mussel
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Abstract
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Hemolysin formed by a psychrophilic bacterium isolated from sea mussel was purified by ammoium sulfate precipitation, DEAE-cellulose column chromatography and sephadex G-100 column chromatography. The hemolysin was 100.7 unit/mg in specific
activity and
22.8 foldconcentrated than the original supernatant, and the yield was 0.3% at the final purification step. The purified hemolysin exhibited the optimum hemolytic activity at near 35℃, but did not active at lower temperatures like below 5℃.
Also
the
thermal stability of the isolated hemolysin was examined. The hemolysin kept 80% for the original hemolytic activity at near 20 ℃ and more residual activity than 95% was kept when treated at lower temperature than 10℃for 10min. Neverthless, the
hemolytic substance was recognized as thermolabile hemolysin bcause lower activity than 5% for the original hemolytic activity was resided when treated at 35℃.
The molecular weight of hemolysin was estimated at approximate 55,000 by using a sephadex G-100 gel filtration.
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